Fluorescence Microscopy Books

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Fluorescence Microscopy


Fluorescence Microscopy
  • Author : Ulrich Kubitscheck
  • Publisher : John Wiley & Sons
  • Release : 2017-05-08
  • ISBN : 9783527338375
  • Language : En, Es, Fr & De
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While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years.

Nanoscopy and Multidimensional Optical Fluorescence Microscopy


Nanoscopy and Multidimensional Optical Fluorescence Microscopy
  • Author : Alberto Diaspro
  • Publisher : CRC Press
  • Release : 2010-04-26
  • ISBN : 9781420078893
  • Language : En, Es, Fr & De
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"Alberto Diaspro has been choreographing light’s dance for over 20 years, and in Nanoscopy and Multidimensional Optical Fluorescence Microscopy, he has assembled a diverse group of experts to explain the methods they use to coax light to reveal biology’s secrets." — From the Foreword by Daniel Evanko, editor, Nature Methods Nanoscopy and Multidimensional Optical Fluorescence Microscopy demonstrates that the boundaries between sciences do blur at the bottom, especially those that might separate the optical work of physicists and the cellular work of microbiologists. In 18 chapters written by pioneering researchers, this work offers the first comprehensive and current documentation of the cutting-edge research being accomplished in a wide range of photonic devices with revolutionary application. The highlight of the book is its coverage of optical nanoscopy and super-resolution microscopy. The rapid advances in this area over the past few years offer researchers in both photonics and molecular biologya wealth of accomplishment upon which they can build. Offering a complete treatment of this emerging field, this volume: Describes how scientists have exploited the properties of light and its fluorophore partners to overcome the resolution limit of conventional light microscopy Delves into recent ways to minimize the photobleaching that has long hampered many methods including those that have the potential to capture previously unobtainable information on the movements of single molecules Discusses the principles, benefits, and implementation of fluorescence correlation spectroscopy and related methods, which simplifies analysis by limiting light to stationary focal points in a sample Considers the most basic as well as emerging methods for improving three-dimensional optical sectioning microscopy Reviews the basics of FRET (fluorescence resonance energy transfer) and considers its new use for investigating protein complexes The text also introduces those emerging nonfluorescence microscopy methods that can actually exert mechanical forces to trap and move a variety of objects ranging from beads to living cells and cellular organelles. Combining this technique with fluorescence microscopy provides an unparalleled ability to manipulate and visualize biological samples. In the half-century since Richard Feynman challenged scientists to come up with the tools to investigate and manipulate our world at the nanoscale, we have succeeded in placing tools in the hands of biophysicists that are leading to major breakthroughs in our understanding of life and our ability to diagnose, treat, and prevent many challenges to human health. This book reflects what has been accomplished to date while pointing the way to what still needs to be done.

Fluorescence Microscopy


Fluorescence Microscopy
  • Author : Brian Herman
  • Publisher : Garland Pub
  • Release : 1998
  • ISBN : 1872748848
  • Language : En, Es, Fr & De
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This volume is a comprehensive revision which combines the theoretical and practical aspects of fluorescence microscopy.

Fluorescence Microscopy


Fluorescence Microscopy
  • Author : Anda Cornea
  • Publisher : Elsevier
  • Release : 2014-02-24
  • ISBN : 9780124167131
  • Language : En, Es, Fr & De
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Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers. With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research. Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical research Offers insight into common challenges in implementing techniques, as well as effective solutions

Fundamentals of Fluorescence Microscopy


Fundamentals of Fluorescence Microscopy
  • Author : Partha Pratim Mondal
  • Publisher : Springer Science & Business Media
  • Release : 2013-12-12
  • ISBN : 9789400775459
  • Language : En, Es, Fr & De
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This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.